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Crimson Taq DNA Polymerase with (Mg-free) Buffer

  • 产品编号:M0325S      品牌:New England Biolabs       原厂货号:M0325S
  • 产品分类:PCR/RT-PCR/qRT-PCR > PCR > 常规PCR
  • 应用分类:
 
包装: 200 units
运保温度: -20°C
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描述:

Description: 
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 
Crimson Taq DNA Polymerase offers superior performance in its newly formulated PCR buffer. Crimson Taq (Mg-free) Reaction Buffer contains a density reagent, which allows direct loading of PCR products onto a gel. In addition, Crimson Taq (Mg-free) Reaction Buffer has a trace amount of a red dye, which serves as an indicator for homogenous reaction setup, a color aid in gel loading and a tracking dye which migrates at about 10 bp on a 1% TBE gel.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Applications:

  • Primer Extension
  • PCR
  • Colony PCR
  • High-throughput PCR

Advantages:

  • Robust and reliable reactions
  • Samples can be loaded directly onto a gel
  • Ideal for high throughput applications

Reagents Supplied: 
Crimson Taq (Mg-free) Reaction Buffer Pack (5X) 
MgCl2 (25 mM)

Heat Inactivation:
No

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Unit Assay Conditions: 1X ThermoPol® Reaction Buffer, 200 μM dNTPs including [3H]-dTTP and 200 μg/ml activated Calf Thymus DNA.

Reaction Conditions: 1X Crimson Taq (Mg-free) Reaction Buffer, DNA template, primers, 200 µM dNTPs (not included), 1.5 mM MgCl2 and 1.25–2.5 units of Crimson Taq DNA Polymerase in a total reaction volume of 50 µl. 

1X Crimson Taq (Mg-free) Reaction Buffer:
12.5 mM Tricine 
42.5 mM KCl
6% Dextran
Acid Red
pH 8.5 @ 25°C

Concentration:
5,000 units/ml

Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C


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