Taq DNA Polymerase with ThermoPol II (Mg-free) Buffer

• 产品编号：M0321S      品牌：New England Biolabs       原厂货号：M0321S
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 常规PCR
• 应用分类：

描述：

• Isolated from a recombinant source

• Supplied with 10X Reaction Buffer

• Robust and reliable reactions

• Tolerates a wide range of templates

• Incorporates dUTP, dITP and fluorescently-labeled nucleotides

• Exceptional value in terms of cost per unit

Description: 
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 

It is supplied with 10X ThermoPol II (Mg-free) Reaction Buffer and MgSO₄. 10X ThermoPol II (Mg-free) Reaction Buffer contains a nonionic detergent to increase enzyme stability during longer incubations.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

Applications:

• PCR
• Primer extension
• Colony PCR

Reagents Supplied: 
MgSO₄ (100 mM)
ThermoPol^® II (Mg-free) Reaction Buffer Pack (10X) 

3´ to 5´ Exonuclease: No
5´ to 3´ Exonuclease: Yes
Strand Displacement: No
Error Rate: 285 x10^-6 bases

Heat Inactivation:
No

Molecular Weight: 
Theoretical: 94,000 kDa

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. 

Unit Assay Conditions: 
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions: 
1X ThermoPol II (Mg-free) Reaction Buffer, DNA template, primers, 200 µM dNTPs (not included), 2 mM MgSO₄ and 1.25 units of Taq DNA Polymerase in a total reaction volume of 50 µl. 

1X ThermoPol II (Mg-free) Reaction Buffer:
20 mM Tris-HCl
10 mM (NH₄)₂S0₄
10 mM KCl 
0.1% Triton X-100
pH 8.8 @ 25°C

Concentration:
5,000 units/ml

Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C

原厂资料：