m7G(5)ppp(5)G RNA Cap Structure Analog

• 产品编号：S1404L      品牌：New England Biolabs       原厂货号：S1404L
• 产品分类：PCR/RT-PCR/qRT-PCR > 其它 > 核苷酸 > 帽相似物&变异型（Cap Analog & Variants）
• 应用分类：

描述：

The 5´terminal m⁷G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

A method for efficient in vitro synthesis of capped RNA using E.coli RNA polymerase primed with m⁷G(5´ )ppp(5´ )G (#S1404S) or m⁷G(5´ )ppp(5´ )A (#S1405S) has been developed by Contreas et al. (7).

Sequence

7mG(5´)ppp(5´)G

Supplied As

Lyophilized Sodium Salt

Suggested Working Concentration

10mM

Recommended Resuspension Volume

100 μl

原厂资料：