Taq 2X Master Mix

• 产品编号：M0270L      品牌：New England Biolabs       原厂货号：M0270L
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 常规PCR
• 应用分类：

描述：

• Robust and reliable reactions

• Tolerates a wide range of templates

•  Incorporates dUTP, dITP and fluorescently-labeled nucleotides

• Exceptional value in terms of cost per unit

Description: 
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5). 
Taq 2X Master Mix is an optimized ready-to-use solution containing Taq DNA Polymerase, dNTPs, MgCl2, KCl, and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It can amplify up to 4 kb from complex genomic DNA or up to 5 kb from lambda DNA.

Source:
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticusYT-1

Applications:

PCR

Primer Extension

Colony PCR

Reagents Supplied: 
MgCl2 (25 mM)

Heat Inactivation:
No

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. 

Unit Assay Conditions:
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 200 µg/ml activated Calf Thymus DNA.

Reaction Conditions:
1X Taq Master Mix, DNA template and primers in a a total reaction volume of 25 or 50 µl.

Taq 1X Master Mix:
10 mM Tris-HCl (pH 8.6 @ 25°C)
50 mM KCl
1.5 mM MgCl2
25 units/ml Taq DNA polymerase
0.2 mM dNTPs each
5% glycerol
0.08% IGEPAL® CA-630
0.05% Tween-20

Concentration:
2 X

原厂资料：