Overview
Ready PCR Mix, 2X is the only PCR master mix that takes you from PCR to Gel to Visualization in one easy step.
• Direct to Gel Loading
• Immediate Visualization
• Safe to Use
Detailed Description
Ready PCR Mix, 2X offers a single-step procedure for performing PCR reactions followed by analysis on agarose gels without the addition of loading buffer. All components for assembly and performance of PCR reactions as well as loading and visualization of PCR products on agarose gels are included. The user supplies primer and template DNA.
Mix includes 2 x 1.25 ml tubes of 2X reaction mix (includes all necessary reagents except template and primer DNA). Combine primers, DNA template, nuclease-free water, and Ready PCR Mix, 2X in a PCR tube. After product amplication, directly load the PCR reactions to a gel and separate. Once electrophoresis is complete, visualize the DNA product using a standard UV illuminator. Sufficient reagents for 50 (1.25ML) or 100 (2X1.25ML) reactions.