NEBuffer 2

产品编号：B7002S 品牌：New England Biolabs 原厂货号：B7002S
产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
应用分类：

包装：

运保温度:

-20°C

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描述：

Description:
New England Biolabs provides a color-coded 10X NEBuffer with each restriction endonuclease to ensure optimal (100%) activity. Most of our enzymes are supplied with one of four standard NEBuffers. Occasionally, an enzyme has specific buffer requirements not met by one of the four standard NEBuffers, in which case the enzyme is supplied with its own unique NEBuffer. Some restriction endonucleases require BSA at a final concentration of 100 µg/ml for optimal activity. When required, BSA is supplied as a 10 mg/ml (100X) stock and should be added to the reaction mixture.

Reagents Supplied:
10X NEBuffer 2
BSA (100X)

1X NEBuffer 2(blue):
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl₂
1 mM Dithiothreitol
pH 7.9 @ 25°C

Quality Control for Current Lot

16-Hour Incubation::
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity::
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.

注意事项：

New England Biolabs provides a color-coded 10X NEBuffer with each restriction enzyme to ensure (100%) optimal activity. The buffer should be used at 1X concentration in the reaction. Some restriction enzymes require bovine serum albumin (BSA) at a final concentration of 100 μg/ml for optimal activity. When required, BSA is supplied as a 10 mg/ml (100X) stock and should be added to the reaction mixture. Restriction enzymes that do not require BSA for optimal activity are not adversely affected if BSA is present in the reaction.

The buffer must be completely thawed before use. Dilute the 10X stock with dH₂O to a final concentration of 1X. Add the water first, buffer next, BSA if required, the DNA solution and finally the enzyme. A typical 50 µl reaction should contain 5 µl of 10X NEBuffer with the rest of the volume coming from the DNA solution, enzyme and dH₂O.