收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

MMESSAGE MMACHINE SP6 KIT 25RXNS

 
包装:
运保温度:
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

其它组分:

Nuclease-free Water【子货号: ,包装:1.75 mL,,运保温度:any temp*】
Enzyme Mix【子货号: ,包装:50 μL,,运保温度:–20°C】
10X Reaction Buffer (【子货号: ,包装:50 μL,,运保温度:–20°C】
2X NTP/CAP【子货号: ,包装:250 μL,,运保温度:–20°C】
GTP† 20 mM in SP6 Kits; 30 mM in T3 and T7 Kits【子货号: ,包装:100 μL,,运保温度:–20°C】
TURBO DNase (2 U/μL)【子货号: ,包装:100 μL,,运保温度:–20°C】
pTRI-Xef, 0.5 mg/mL (Control Template)【子货号: ,包装:10 μL,,运保温度:–20°C】
Ammonium Acetate Stop Solution【子货号: ,包装:1 mL,,运保温度:–20°C】
Lithium Chloride Precipitation Solution【子货号: ,包装:1.4 mL,,运保温度:–20°C】
Gel Loading Buffer II【子货号: ,包装:1.4 mL,,运保温度:–20°C】


描述:

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 μg of 7-methyl guanosine capped RNA from 1 μg of template DNA. Each kit includes sufficient reagents for 25 reactions.

• High yields of capped RNA
• Extra convenience; cap analog-nucleotide mix provided
• Simplified reaction format
• Synthesized transcripts ideal for microinjection, in vitro translation, or transfection

In a 20 μl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield large mass amounts of capped RNA. Up to 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 μg) and proportion of transcripts that are capped (80%). mMESSAGE mMACHINE® kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation.

mMESSAGE mMACHINE® Kits are only optimized for use with the polymerases included in the kit. Using a different polymerase than what is supplied in the kit may result in low yields. Each mMESSAGE mMACHINE® Kit contains 38 U of cap analog, an outstanding value for the cost.


相关图片

本产品可用于的实验

最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错