硫酸钠葡聚糖(DSS)Dextran Sulfate Sodium Salt (36,000-50,000 M.Wt.) Colitis Grade

• 产品编号：0216011050      品牌：MP       原厂货号：0216011050
• 产品分类：生化试剂 > 生化试剂 > 常用化学试剂
• 应用分类：行业及应用 > 药物研发 > 结肠炎造模

描述：

Dextran sulfate sodium salt
 

Sodium Salt
Synonyms: Dextran Sodium Sulfate

Structure:



CAS # : 9011-18-1

Physical Description: White to off-white powder

Description: Dextran sulfate is a polyanionic derivative of dextran produced by esterification of Dextran with chlorosulphonic acid. The sulfur content is approximately 17% which corresponds to an average of 1.9 sulfate groups per glucosyl residue of the dextran molecule.

Typical Uses:

• • Increases hybridization rate of nucleic acids - in the presence of 10% dextran sulfate the rate of reannealing of DNA in solution was increased by about 10 times.¹⁵ This observation was later extended to the hybridization of single or double-stranded probes to DNA or RNA immobilized on filter paper.^9,16 The addition of 10% dextran sulfate may increase the rate of hybridization of randomly cleaved double-stranded DNA probes to immobilized nucleic acids by as much as 100 times.¹⁶
  • Shows immunologically relevant activities:
    • 1. enhancement and suppression of humoral immunity^3,4
      2. polyclonal activation of B-lymphocytes, stimulating even immature B cells⁶
      3. changes in thymocyte reactivity to lectins¹
      4. inhibition of blood coagulation and platelet aggregation¹¹
      5. enhancement of blood fibrinolytic activity¹¹
      6. enhancement/suppression of cell-mediated immune responses.^2,10
  • Precipitates LDL and VLDL lipoproteins - in the presence of magnesium ions, dextran sulfate precipitates low-density lipoproteins from human serum leaving the high-density lipoproteins in the supernatant. Removal of lipoproteins by dextran sulfate precipitation may be useful in the purification of other materials such as beta2-glycoprotein.⁸
  • Shows activity as an adjuvant

Availability:

Solubility: Soluble in water (100 mg/ml - clear to slightly hazy yellow solution. Higher molecular weight products may not be as soluble in water as the lower molecular weight products.). Aqueous solutions should be buffered with (e.g. with sodium bicarbonate) before autoclaving to prevent decomposition.¹⁷ 

References:
 

1. Blitstein-Willinger, E., Schutz, G. and Diamanststein, T., "Changes in thymocyte reactivity to lectins by B-cell mitogens of the type of sulphated polyanions." Immunology, v. 30, 529-533 (1976).
2. Bradfield, J.W.B., Souhami, R.L., Addison, J.E., "The mechanism of the adjuvant action of dextran sulphate." Immunology, v. 26, 383-386 (1974).
3. Diamantstein, T., et al., "Stimulation of humoral antibody formation by polyanions. II. The influence of sulphate esters of polymers on the immune response on mice." Eur. J. Immunol., v. 1, 340-343 (1971).
4. Diamantstein, T., et al., "Suppression of the primary immune response in vivo to sheep red blood cells by B-cell mitogens." Immunology,v. 30, 401-405 (1976).
5. Finley, P.R., et al., "Cholesterol in high-density lipoprotein: Use of Mg⁺²/dextran sulphate in its enzymatic measurement." Clin. Chem.,v. 24, 931-933 (1978).
6. Granstrom, M., et al., "The polyclonal B-cell activator Dextran sulphate induces formation of colony stimulating activity." Scand. J. Immunol., v. 7, 277-284 (1978).
7. Laderman, L., Kawasaki, E.S and Szabo, P., "The rate of nucleic acid annealing to cytological preparation is increased in the presence of dextran sulphate." Anal. Biochem., v. 117, 158-163 (1981).
8. Lambin, P. and Burstein, M., "Isolation of a beta2-glycoprotein from human serum after precipitation with dextran sulphate and manganese chloride." Biochimie, v. 64, 1065-1071 (1982).
9. Maniatis, T., Fritsch, E. and Sambrook, F. (eds.), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory (1982).
10. McCarthey, R.E. and Babcock, G.F., "Simultaneous stimulation and suppression of two different indicators of the cell-mediated immune response by the immunoregulator dextran sulphate." Immunology, v. 34, 827-929 (1978).
11. Nakajima, K., et al., "Effects of dextran sulphate on blood coagulation and fibrinolysis in spontaneously hypertensive rats." Adv. Inflamm. Res., v. 1, 331-337 (1979).
12. Ricketts, C.R., "Dextran Sulphate - A synthetic analogue of Heparin." Biochemistry, v. 51, 129-133 (1952).
13. Ricketts, C.R., "A basic derivative of dextran and its interaction with serum albumin." Biochem. J., v. 76, 117-120 (1960).
14. Warnick, G.R., et al., "HDL cholesterol quantitation by phosphotungstate - Mg²⁺ and by dextran sulphate - Mn²⁺ - polyethylene glycol precipitation, both with enzymatic cholesterol assay compared with the lipid research method." Amer. J. Clin. Pathol., v. 78, 718-723 (1982).
15. Wetmur, J.G., "Acceleration of DNA renaturation rates." Biopolymers, v. 14, 2517-2524 (1975).
16. Wahl, G.M., et al., "Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization with dextran sulfate." Proc. Natl. Acad. Sci. USA, v. 76, 3683 (1979).
17. Merck Index, 12th Ed., No. 2993.

18. 描 述：硫酸葡聚糖是葡聚糖的聚阴离子衍生物，由葡聚糖和氯磺酸的酯化反应形成。其中含硫量约为17%，相当于葡聚糖分子的每个葡萄糖残糖中平均含1.9个硫酸基团。

    用途：提高核酸杂交率 、 显示免疫相关的活动、 沉淀低密度（LDL）和极低密度（VDLD）脂蛋白、作为佐剂发挥作用、结肠炎造模。

    结肠炎造模：

    • 方法一：小鼠给予含50 g/L硫酸葡聚糖(葡聚糖硫酸钠) 5000的蒸馏水自由饮用7 d，出现体重减轻、腹泻、血便等急性肠炎的表现.病理学切片HE染色发现小鼠病变结肠腺体结构紊乱，黏膜和黏膜下单核细胞和多核细胞浸润.DSS组病变肠段组织GSH表达较对照组明显减少
    • 方法二：将分子量为50 00的DSS溶于饮用水，配成3% DSS溶液，让小鼠自由饮用7d，建立小鼠急性结肠炎模型;② 让小鼠自由饮用3% DSS溶液7d，然后饮用不含DSS蒸馏水14d，建立小鼠慢性结肠炎模型;

    该模型的优点主要有:
    1.日本学者于1985年首次应用DSS制备了鼠UC模型，发现此模型的病理改变与人类UC最相似;
    2.此模型既可以应用于UC急性期又可应用于慢性期的研究，使实验能够完整地进行;
    3.制作简便、经济、易于复制

原厂资料：