葡聚糖硫酸酯Dextran Sulfate Sodium Salt (1,400,000 Avg. M.Wt)

• 产品编号：0219399210      品牌：MP       原厂货号：0219399210
• 产品分类：生化试剂 > 化学试剂 > 有机溶剂
• 应用分类：行业及应用 > 药物研发 > 结肠炎造模

描述：

Dextran sodium sulfate

Molecular Biology Reagent
Sodium Salt
Ideal for nucleic acid hybridizations

Description: Dextran sulfate is a polyanionic derivative of dextran produced by esterification of Dextran with chlorosulphonic acid. The sulfur content is approximately 17% which corresponds to an average of 1.9 sulfate groups per glucosyl residue of the dextran molecule.

Typical Uses:
 

• • Increases hybridization rate of nucleic acids - in the presence of 10% dextran sulfate the rate of reannealing of DNA in solution was increased by about 10 times.¹⁵ This observation was later extended to the hybridization of single or double-stranded probes to DNA or RNA immobilized on filter paper.^9,16 The addition of 10% dextran sulfate may increase the rate of hybridization of randomly cleaved double-stranded DNA probes to immobilized nucleic acids by as much as 100 times.¹⁶
  • Shows immunologically relevant activities:
    • 1. enhancement and suppression of humoral immunity^3,4
      2. polyclonal activation of B-lymphocytes, stimulating even immature B cells⁶
      3. changes in thymocyte reactivity to lectins¹
      4. inhibition of blood coagulation and platelet aggregation¹¹
      5. enhancement of blood fibrinolytic activity¹¹
      6. enhancement/suppression of cell-mediated immune responses.^2,10
  • Precipitates LDL and VLDL lipoproteins - in the presence of magnesium ions, dextran sulfate precipitates low-density lipoproteins from human serum leaving the high-density lipoproteins in the supernatant. Removal of lipoproteins by dextran sulfate precipitation may be useful in the purification of other materials such as beta2-glycoprotein.

硫酸钠葡聚糖；硫酸葡聚糖钠盐；葡聚糖硫酸酯钠盐；葡聚糖硫酸钠

用途：

·提高核酸杂交率 - 溶液中含10%的硫酸葡聚糖，DNA链的再退火率约增加10倍，这一现象进一步扩大了单链或双链的探针与固定在膜上的DNA/RNA的杂交率。不仅如此，添加10%硫酸葡聚糖也许会增加随机切割的双链DNA探针与固定化核酸的杂交率，高达100倍。

·显示免疫相关的活动：

（1）体液免疫的增强和抑制；

（2）B淋巴细胞的多克隆活化作用，甚至可以刺激未成熟的B细胞；

（3）胸腺细胞反应中，促使转化成凝集素；

（4）抑制血液凝固和血小板聚集；

（5）增强血纤维蛋白溶解活性；

（6）增强/抑制细胞介导的免疫应答；

·沉淀低密度（LDL）和极低密度（VDLD）脂蛋白 -利用硫酸葡聚糖的沉淀作用去除脂蛋白这一功能同样可以用来纯化其他材料，如β-2-糖蛋白。

·作为佐剂发挥作用

原厂资料：

注意事项：

Soluble in water (100 mg/ml - clear to slightly hazy yellow solution.Higher molecular weight products may not be as soluble in water as the lower molecular weight products.). Aqueous solutions should be buffered with (e.g. with sodium bicarbonate) before autoclaving to prevent decomposition.