AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. Features of this enzyme:
AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
Its thermal activity profile makes it good for PCR applications
It is QC-tested to guarantee reproducible results
Good for PCR
The thermal activity profile of AmpliTaq® DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer II. It is also available with GeneAmp® 10X PCR Buffer I and MgCl2 Solution.
Also available is AmpliTaq® 360 DNA Polymerase for superior PCR results with the broadest range of templates. Compared to the original AmpliTaq® DNA Polymerase, AmpliTaq® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultrapure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq® 360 Buffer Kit, promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to help work through challenging GC-rich templates.
内容和储存
Contents:
• 5 × 200 µL of AmpliTaq® DNA Polymerase (5 U/µL)
• 4 × 1.5 mL of GeneAmp® 10X PCR Buffer II (500 mM KCl, 100 mM Tris-HCl pH 8.3)
• 4 × 1.5 mL of 25 mM MgCl2.
Store at -20°C.
原厂资料：
AmpliTaq® DNA Polymerase is a 94 kDa, thermostable, recombinant DNA polymerase obtained by expression of a modified form of the Thermus aquaticus (Taq) DNA polymerase gene in E. coli. It is the most thoroughly characterized enzyme available for the PCR process and its recombinant nature and purification method provide unparalleled purity and reproducibility, vial-to-vial, lot-to-lot. Features of this enzyme:
AmpliTaq® DNA Polymerase is the most thoroughly characterized enzyme available for the PCR process, a testimony to its overall utility and efficacy
Its thermal activity profile makes it good for PCR applications
It is QC-tested to guarantee reproducible results
Good for PCR
The thermal activity profile of AmpliTaq® DNA Polymerase is good for PCR applications because its optimal activity is in the same range at which stringent annealing of primers occurs (55–75°C). The enzyme's half-life is ~40 minutes at 95°C, providing thermostability that meets the requirements of most difficult PCR applications. This AmpliTaq® DNA Polymerase is supplied with GeneAmp® 10X PCR Buffer II. It is also available with GeneAmp® 10X PCR Buffer I and MgCl2 Solution.
Also available is AmpliTaq® 360 DNA Polymerase for superior PCR results with the broadest range of templates. Compared to the original AmpliTaq® DNA Polymerase, AmpliTaq® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. This ultrapure enzyme reduces false positive results, amplifies low-level target sequences, and when combined with the proprietary AmpliTaq® 360 Buffer Kit, promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to help work through challenging GC-rich templates.
内容和储存
Contents:
• 5 × 200 µL of AmpliTaq® DNA Polymerase (5 U/µL)
• 4 × 1.5 mL of GeneAmp® 10X PCR Buffer II (500 mM KCl, 100 mM Tris-HCl pH 8.3)
• 4 × 1.5 mL of 25 mM MgCl2.
Store at -20°C.
注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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