TAQMAN PROTEIN EXPN COREREAGENTS KIT

• 产品编号：4405501      品牌：invitrogenGA       原厂货号：4405501
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > qPCR蛋白检测
• 应用分类：

描述：

Applied Biosystems® TaqMan® Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan® Protein Expression Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression assays. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems® real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan® Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan® Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry. Key features of the TaqMan® Protein Expression Assay: 
• Quantitates protein expression using gold standard TaqMan® 5’ nuclease chemistry 
• Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform 
• Allows greater sensitivity and a more quantitative readout than western blots and immunostaining.

原厂资料：

Applied Biosystems® TaqMan® Protein Expression Assays enable fast, easy identification, and relative protein quantification of pluripotency protein markers from limited quantities of cultured hESCs. The TaqMan® Protein Expression Assays Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression assays. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems® real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan® Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan® Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry. Key features of the TaqMan® Protein Expression Assay: 
• Quantitates protein expression using gold standard TaqMan® 5’ nuclease chemistry 
• Enables direct correlation of mRNA and/or miRNA expression to protein expression on the same real-time PCR platform 
• Allows greater sensitivity and a more quantitative readout than western blots and immunostaining.

注意事项：

For Research Use Only. Not for use in diagnostic procedures.