Hu CD8 T Lym Enrichment IMag-DM Set

• 产品编号：BD-557941      品牌：BD-Pharmingen       原厂货号：557941
• 产品分类：生化和细胞分析 > 微珠和磁珠 > 细胞分选磁珠 > 分离人细胞
• 应用分类：

描述：

The BD™ IMag Human CD8 T Lymphocyte Enrichment Set - DM is used for the negative selection of CD8 T lymphocytes from peripheral blood. The Biotinylated Human CD8 T Lymphocyte Enrichment Cocktail contains monoclonal antibodies that recognize antigens expressed on erythrocytes, platelets, and peripheral leukocytes that are not CD8 T lymphocytes. The BD™ IMag Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. With these two components, the BD IMag Human CD8 T Lymphocyte Enrichment Set -DM avoids the inadvertent activation of the enriched CD8 T lymphocytes by using reagents that do not directly bind to those T cells. This Enrichment Set has been optimized for use with the BD™ IMagnet, and it contains sufficient reagents to label 10^9 peripheral blood mononuclear cells (PBMC).

The detailed Magnetic Labeling and Enrichment Protocol follows. In summary, the Biotinylated Human CD8 T Lymphocyte Enrichment Cocktail simultaneously stains erythrocytes, platelets, and most leukocytes except the CD8 T lymphocytes. After washing away excess antibody, BD IMag Streptavidin Particles Plus - DM are added to the cell suspension and bind the cells bearing the biotinylated antibodies. The tube containing this labeled cell suspension is then placed within the magnetic field of the BD IMagnet (Cat. No. 552311). Negative selection is then performed to enrich for the unlabeled T cells. Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off and retained (enriched fraction). The negative selection is repeated twice to increase the yield of the enriched fraction. If greater purity is required, negative selection may be performed on the enriched fraction. For clarification of the procedure, the magnetic separation steps are diagrammed in the Enrichment Flow Chart. The enriched (and positive) fractions can be evaluated in downstream applications such as flow cytometry and tissue culture. The antibodies in the Biotinylated Human CD8 T Lymphocyte Enrichment Cocktail have been optimized and pre-diluted to provide maximum efficiency for enrichment of CD8 T lymphocytes from PBMC.

The CD8 T Lymphocyte Enrichment Cocktail is comprised from the following biotin-conjugated monoclonal antibodies:

Anti-human CD4, clone L200

Anti-human CD11b/Mac-1 (CR3), clone ICRF44

Anti-human CD16, clone 3G8

Anti-human CD19, clone HIB19

Anti-human CD36, clone CB38 (NL07)

Anti-human CD41a, clone HIP8

Anti-human CD56, clone B159

Anti-human CD123 (IL-3 Receptor α chain), clone 9F5

Anti-human CD235a (Glycophorin A), clone GA-R2 (HIR2)

Anti-human γδ TCR, clone B1

原厂资料：

The BD™ IMag Human CD8 T Lymphocyte Enrichment Set - DM is used for the negative selection of CD8 T lymphocytes from peripheral blood. The Biotinylated Human CD8 T Lymphocyte Enrichment Cocktail contains monoclonal antibodies that recognize antigens expressed on erythrocytes, platelets, and peripheral leukocytes that are not CD8 T lymphocytes. The BD™ IMag Streptavidin Particles Plus - DM are magnetic nanoparticles that have streptavidin covalently conjugated to their surfaces. With these two components, the BD IMag Human CD8 T Lymphocyte Enrichment Set -DM avoids the inadvertent activation of the enriched CD8 T lymphocytes by using reagents that do not directly bind to those T cells. This Enrichment Set has been optimized for use with the BD™ IMagnet, and it contains sufficient reagents to label 10^9 peripheral blood mononuclear cells (PBMC).

The detailed Magnetic Labeling and Enrichment Protocol follows. In summary, the Biotinylated Human CD8 T Lymphocyte Enrichment Cocktail simultaneously stains erythrocytes, platelets, and most leukocytes except the CD8 T lymphocytes. After washing away excess antibody, BD IMag Streptavidin Particles Plus - DM are added to the cell suspension and bind the cells bearing the biotinylated antibodies. The tube containing this labeled cell suspension is then placed within the magnetic field of the BD IMagnet (Cat. No. 552311). Negative selection is then performed to enrich for the unlabeled T cells. Labeled cells migrate toward the magnet (positive fraction), leaving the unlabeled cells in suspension so they can be drawn off and retained (enriched fraction). The negative selection is repeated twice to increase the yield of the enriched fraction. If greater purity is required, negative selection may be performed on the enriched fraction. For clarification of the procedure, the magnetic separation steps are diagrammed in the Enrichment Flow Chart. The enriched (and positive) fractions can be evaluated in downstream applications such as flow cytometry and tissue culture. The antibodies in the Biotinylated Human CD8 T Lymphocyte Enrichment Cocktail have been optimized and pre-diluted to provide maximum efficiency for enrichment of CD8 T lymphocytes from PBMC.

The CD8 T Lymphocyte Enrichment Cocktail is comprised from the following biotin-conjugated monoclonal antibodies:

Anti-human CD4, clone L200

Anti-human CD11b/Mac-1 (CR3), clone ICRF44

Anti-human CD16, clone 3G8

Anti-human CD19, clone HIB19

Anti-human CD36, clone CB38 (NL07)

Anti-human CD41a, clone HIP8

Anti-human CD56, clone B159

Anti-human CD123 (IL-3 Receptor α chain), clone 9F5

Anti-human CD235a (Glycophorin A), clone GA-R2 (HIR2)

Anti-human γδ TCR, clone B1

注意事项：