Hu IL-2 Recom 10ug

产品编号：BD-554603 品牌：BD-Pharmingen 原厂货号：554603
产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > IL-2 Family 白细胞介素-2超家族
应用分类：细胞培养产品与服务 > 哺乳动物细胞培养 > 生长因子和纯化蛋白质 > 物种 > 人
　　　　　细胞培养产品与服务 > 哺乳动物细胞培养 > 细胞因子 > 白细胞介素

包装：

10ug

运保温度:

Store product at -80°C prior to use or for long te

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描述：

Interleukin-2 (IL-2), originally called T cell growth factor (TCGF), is a multifunctional cytokine which can stimulate the differentiation and proliferation of T lymphocytes and other cell types including B lymphocytes, NK cells, LAK cells, and monocytes/macrophages. IL-2 exerts its biological effects by binding to specific receptors expressed by various target cells. Human IL-2 is a 15 kD protein containing 133 amino acid residues.

Formulation and Purity

Recombinant human IL-2 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, pH 7.2, and containing 2.0 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The human IL-2 was purified by immunoaffinity chromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law. The endotoxin level is ≤ 0.1 ng per µg of human IL-2, as measured in a chromogenic LAL assay.

Preparation and Storage

Store product at -80°C prior to use or for long term storage of stock solutions.This preparation contains no preservatives, thus it should be handled under aseptic conditions.Avoid multiple freeze-thaws of product.Rapidly thaw and quick-spin product prior to use.Upon initial thawing, the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/mL carrier protein such as human or bovine serum albumin, aliquoted and stored at -80°C. For in vitro biological assay use, carrier-protein concentrations of > 1 mg/mL are suggested. For use as an ELISA standard, a carrier-protein concentrations of 5 - 10 mg/mL is suggested.

注意事项：

Store product at -80°C prior to use or for long term storage of stock solutions.
This preparation contains no preservatives, thus it should be handled under aseptic conditions.
The product can be reconstituted in sterile neutral buffer containing 0.5 - 10 mg/ml carrier protein such as human or bovine
serum albumin, aliquoted and stored at –80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of
0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier
protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible
effects in an appropriate experimental system. Carrier proteins may affect experimental results due to toxicity, high endotoxin
levels or possible blocking activity.