Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb

• 产品编号：CST-12390S      品牌：CST       原厂货号：12390S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb仅仅能够检测当Ser477发生磷酸化时内源性水平的IRF-7蛋白。这种抗体同样可以检测Ser477和Ser479同时磷酸化时的IRF-7。这种抗体会与100和150kDa处的未知来源的蛋白发生交叉反应。此单克隆抗体通过合成肽免疫动物制备，这种合成肽是人源IRF-7蛋白Ser477周围的肽段。干扰素调控因子(IRFs) 包含一个家族的转录调控因子，这个家族主要是在 Jak/Stat信号通路中起作用，在受到病毒的感染后能够调控干扰素(IFN)和干扰素诱导的基因表达(1)。IRFs在抵抗病原菌、自体免疫、淋巴细胞发育、细胞生长和易感性的转变等过程中发挥了重要的作用。IRF家族包含9个成员: IRF-1、IRF-2、ISGF3γ/p48、IRF-3、 IRF-4 (Pip/LSIRF/ICSAT)、 IRF-5、 IRF-6、 IRF-7和IRF-8/ICSBP。所有的IRF蛋白在N-末端的DNA-结合区域都具有同源性。IRF家族成员通过与具有相似DNA-结合区域（例如IFN刺激的应答原件(ISRE)、IFN consensus序列 (ICS) 和IFN 调控元件(IRF-E)） 的蛋白相互作用而调控转录(2)。IRF-7在功能上与IRF-3相似,优先在淋巴样细胞中表达，并且能被病毒、LPS 和 IFN-α所诱导(3-5)。受到病毒感染后，IRF-7在诱导I型干扰素表达方面发挥了重要作用(6-8)。与IRF-3相似, IRF-7的调控是通过在C-末端附近多个丝氨酸位点磷酸化而实现，这对其核定位、DNA结合和转录活性都是必需的(9-11)。

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Au, W.C. et al. (1998) J Biol Chem 273, 29210-7.
4. Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18.
5. Marié, I. et al. (1998) EMBO J 17, 6660-9.
6. Sato, M. et al. (2000) Immunity 13, 539-48.
7. Honda, K. et al. (2005) Nature 434, 772-7.
8. Colina, R. et al. (2008) Nature 452, 323-8.
9. Lin, R. et al. (2000) J Biol Chem 275, 34320-7.
10. Yang, H. et al. (2003) J Biol Chem 278, 15495-504.
11. Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-IRF-7 (Ser477) (D7E1W) Rabbit mAb recognizes endogenous levels of IRF-7 protein only when phosphorylated at Ser477. This antibody can also detect IRF-7 when dually phosphorylated at Ser477 and Ser479. This antibody may cross-react with proteins of unknown origin between 100 and 150 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser477 of human IRF-7 protein.

Background

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, ISGF3γ/p48, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).IRF-7, which is functionally similar to IRF-3, is preferentially expressed in lymphoid cells and induced by virus, LPS, and IFN-α (3-5). IRF-7 plays an essential role in the induction of type I interferon in response to viral infection (6-8). Like IRF-3, IRF-7 is regulated at multiple serine phosphorylation sites near its carboxyl terminus, which are required for nuclear translocation, DNA binding, and transcriptional activity (9-11)

1. Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55.
2. Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58.
3. Au, W.C. et al. (1998) J Biol Chem 273, 29210-7.
4. Wathelet, M.G. et al. (1998) Mol Cell 1, 507-18.
5. Marié, I. et al. (1998) EMBO J 17, 6660-9.
6. Sato, M. et al. (2000) Immunity 13, 539-48.
7. Honda, K. et al. (2005) Nature 434, 772-7.
8. Colina, R. et al. (2008) Nature 452, 323-8.
9. Lin, R. et al. (2000) J Biol Chem 275, 34320-7.
10. Yang, H. et al. (2003) J Biol Chem 278, 15495-504.
11. Caillaud, A. et al. (2005) J Biol Chem 280, 17671-7.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.