PI/RNase Staining Buffer Solution 100mL

• 产品编号：BD-550825      品牌：BD-Pharmingen       原厂货号：550825
• 产品分类：生化试剂 > 染料/染色剂/指示剂/底物 > 细胞染料 > 细胞核酸染料
• 应用分类：

描述：

碘化丙啶（PI）是一种荧光活体染料，可用来染色DNA和RNA。PI可结合到DNA和RNA，所以后者必须要用核糖核酸酶消化。流式细胞仪分析出的DNA成份可揭示细胞周期信息及涉及细胞周期调节的蛋白质信息。在G2期和M期的DNA为G0期和G1期的两倍。S期细胞所含DNA量介于两者之间。PI检测在橙波范围，使用562-588L nm 带通滤波器检测。PI可用于流式细胞仪分析细胞周期，还可与抗体结合作用检测细胞周期中蛋白质表达。试剂悬于磷酸盐缓冲液中（PBS），pH 7.2，含0.02%(w/v)叠氮化钠。使用时不需要稀释。每次检测使用0.5 ml (1 x 106个细胞)，检测前室温孵育15分钟。

原厂资料：

Propidium Iodide (PI) is a fluorescent vital dye that stains DNA and RNA. PI binds to both DNA and RNA, so the latter must be removed by digestion with ribonucleases. The content of DNA as determined by flow cytometry, can reveal useful information about the cell cycle and the proteins involved in the regulation of the cell cycle. Cells in G2 and M phases of the cell cycle have double the DNA content of those in G0 and G1 phases. Cells in S phase have a DNA content lying between these extremes. PI is detected in the orange range of the spectrum using a 562-588 nm band pass filter. This reagent may be used to analyze cell cycle by flow cytometry in addition to use with antibodies for examining the expression of proteins during the cell cycle.

注意事项：

1.Avoid contact with skin and eyes.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.Do not dilute or boil.