Active Caspase-3 Apop Kit FITC 100Tst

• 产品编号：BD-550480      品牌：BD-Pharmingen       原厂货号：550480
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞凋亡试剂盒
• 应用分类：

原厂资料：

The caspase family of cysteine proteases plays a key role in apoptosis and inflammation. Caspase-3 is a key protease that is activated during
the early stages of apoptosis and, like other members of the caspase family, is synthesized as an inactive pro-enzyme that is processed in cells
undergoing apoptosis by self-proteolysis and/or cleavage by another protease. The processed forms of caspases consist of large (17-22 kDa)
and small (10-12 kDa) subunits which associate to form an active enzyme. Active caspase-3, a marker for cells undergoing apoptosis, consists
of a heterodimer of 17 and 12 kDa subunits which is derived from the 32 kDa pro-enzyme. Active caspase-3 proteolytically cleaves and
activates other caspases, as well as relevant targets in the cytoplasm, e.g., D4-GDI and Bcl-2, and in the nucleus (e.g. PARP). This antibody
has been reported to specifically recognize the active form of caspase-3 in human and mouse cells. It has not been reported to recognize the
pro-enzyme form of caspase-3.

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.