pMAL-c5X Vector

产品编号：N8108S 品牌：New England Biolabs 原厂货号：N8108S
产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 细菌表达载体
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包装：

运保温度:

-20°C

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描述：

The vector pMAL-c5X is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Factor Xa (NEB #P8010). MBP fusions made with this vector are expressed cytoplasmically. The MBP has been engineered for tighter binding to amylose resin.

A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding maltose-binding protein). The fusion protein thus produced can be purified by amylose affinity chromatography. The sequence coding for the four amino acids Ile-Glu-Gly-Arg is present just upstream of the XmnI site. This allows the protein of interest to be cleaved from maltose-binding protein with the specific protease Factor Xa. Fragments inserted in the XmnI site (cleaves GAAGG↓ATTTC) will produce a fusion protein that, after Factor Xa cleavage, contains no vector-derived residues on the protein of interest.

Source:
NEB 10-beta Competent E. coli (pMAL-c5X)

Concentration:
200 μg/ml

Storage Conditions:
10 mM Tris-HCl
1 mM EDTA

Affinity Tag

Maltose-Binding Protein (MBP)

注意事项：

NEB 10-beta Competent E. coli (High Efficiency) (NEB #C3019) is recommended for propagation and subcloning. NEB Express Competent E.coli (High Efficiency) (NEB #C2523) is recommended for expression using this vector.