pMAL-c5E Vector

• 产品编号：N8110S      品牌：New England Biolabs       原厂货号：N8110S
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 细菌表达载体
• 应用分类：

描述：

The vector pMAL-c5E is designed to produce maltose-binding protein (MBP) fusions, where the protein of interest can be cleaved from MBP with the specific protease Enterokinase (NEB #P8070). 
MBP fusions made with this vector are expressed cytoplasmically. The MBP has been engineered for tighter binding to amylose resin.
A gene or open reading frame is inserted into a restriction site of the vector polylinker, in the same translational reading frame as the malE gene (encoding MBP). The fusion protein produced from the vector can be purified by amylose affinity chromatography. The sequence coding for the five amino acids Asp-Asp-Asp-Asp-Lys is present just upstream of the KpnI site. This allows the protein of interest to be cleaved from maltose-binding protein with enterokinase. 

Affinity Tag

Maltose-Binding Protein (MBP)

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ -20°C

原厂资料：