Phospho-RanBP3 (Ser58) Antibody

• 产品编号：CST-9380S      品牌：CST       原厂货号：9380S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-RanBP3 (Ser58) Antibody检测仅在Ser58位点磷酸化的内源性RanBP3b蛋白和仅在Ser126位点发生磷酸化的RanBP3a蛋白。通过人工合成人源RanBP3b蛋白Ser58位点相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。RanBP3蛋白起初被鉴定作为RanGTP结合蛋白，其定位于细胞核并且涉及细胞核的输出过程(1)。它的功能通过结合CRM1作为CRM1细胞核输出的辅助因子，从而稳定RanGTP-CRM1-cargo的相互作用和促进细胞核中心蛋白复合物的关联(2,3)。在Ran-bound GTP缺少的情况下，RanBP3蛋白抑制CRM1复合物结合到细胞核中心复合物。除了CRM1之外，RanBP3蛋白也已经被证实结合到RanGEF-RCC1，并且对于RanGTP-CRM1-Cargo可增加RCC1蛋白的鸟嘌呤核苷酸交换活性(1,4)。在某些情况下，正如 β-catenin和Smad2/3，RanBP3结合可能一种Ran依赖而CRM1非依赖的方式去介导靶蛋白的细胞核输出(5,6)。通过PI3K/Akt或ERK/RSK通路使RanBP3蛋白在Ser58位点磷酸化。该磷酸化在细胞核输出中对RanBP3蛋白功能起着重要作用，很可能是由于RCC1蛋白活性的刺激(7,8)。

1. Mueller, L. et al. (1998) FEBS Lett 427, 330-6.
2. Lindsay, M.E. et al. (2001) J Cell Biol 153, 1391-402.
3. Englmeier, L. et al. (2001) EMBO Rep 2, 926-32.
4. Nemergut, M.E. et al. (2002) J Biol Chem 277, 17385-8.
5. Hendriksen, J. et al. (2005) J Cell Biol 171, 785-97.
6. Dai, F. et al. (2009) Dev Cell 16, 345-57.
7. Yoon, S.O. et al. (2008) Mol Cell 29, 362-75.
8. von Knethen, A. et al. (2010) J Cell Sci 123, 192-201.

原厂资料：

Specificity / Sensitivity

Phospho-RanBP3 (Ser58) Antibody recognizes endogenous levels of RanBP3b protein only when phosphorylated at Ser58, and RanBP3a protein only when phosphorylated at corresponding residue Ser126.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser58 of human RanBP3b protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

RanBP3 was originally identified as RanGTP binding protein located in the nucleus and involved in the nuclear exporting process (1). It functions as a cofactor for CRM1 nuclear export by binding to CRM1, stabilizing the RanGTP-CRM1-cargo interaction and promoting complex association with nuclear pore proteins (2,3). In the absence of Ran-bound GTP, RanBP3 prevents binding of CRM1 complex to the nuclear pore complex. In addition to CRM1, RanBP3 also has been shown to bind to RanGEF-RCC1 and increase the guanine nucleotide exchange activity of RCC1 for RanGTP-CRM1-Cargo (1,4). In some cases, as with β-catenin and Smad2/3, RanBP3 binding may mediate the target protein nuclear export in a Ran-dependent, but CRM1-independent manner (5,6). RanBP3 is phosphorylated at Ser58 through the PI3K/Akt or ERK/RSK pathway. This phosphorylation is important for RanBP3 function in nuclear export, likely due to stimulation of RCC1 activity (7,8).

1. Mueller, L. et al. (1998) FEBS Lett 427, 330-6.
2. Lindsay, M.E. et al. (2001) J Cell Biol 153, 1391-402.
3. Englmeier, L. et al. (2001) EMBO Rep 2, 926-32.
4. Nemergut, M.E. et al. (2002) J Biol Chem 277, 17385-8.
5. Hendriksen, J. et al. (2005) J Cell Biol 171, 785-97.
6. Dai, F. et al. (2009) Dev Cell 16, 345-57.
7. Yoon, S.O. et al. (2008) Mol Cell 29, 362-75.
8. von Knethen, A. et al. (2010) J Cell Sci 123, 192-201.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.