Troponin T (Cardiac) Antibody

• 产品编号：CST-5593S      品牌：CST       原厂货号：5593S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Troponin T (Cardiac) Antibody检测内源性心脏的Troponin T总蛋白。通过人工合成人源心脏的Troponin T蛋白Pro69位点周围相应的多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Troponin蛋白连同tropomyosin蛋白一起发挥作用，该蛋白作为一个分子开关，在细胞内Ca2+浓度变化的情况下调节肌肉收缩。Troponin蛋白有三个亚型组成：Ca2+结合亚基troponin C (TnC)、肌球蛋白结合亚基troponin T (TnT)和抑制亚基troponin I (TnI)(1)。在心脏的β-adrenergic刺激下，TnI (cardiac)的Ser23和Ser24位点是通过PKA 和PKC 磷酸化。这个磷酸化刺激TnC蛋白的调节区域的构象变化，减少TnI和TnC的联系，并且通过减少肌丝Ca2+敏感度从而减少TnC蛋白的Ca2+结合度(1-3)。troponin复合物的原肌球蛋白结合亚基TnT存在三种不同的亚型：慢骨骼肌亚型(ssTnT/TNNT1)、快骨骼肌亚型(fsTnT/TNNT3)和心肌亚型(cTnT/TNNT2)。这些中的每一个也可能包含杜仲选择性剪切体。cTnT和cTnI蛋白的血清浓度检测分析已经在分析心肌损伤中被报道。

1. Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
2. Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
3. Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Troponin T (Cardiac) Antibody detects endogenous levels of total cardiac Troponin T protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a region surrounding Pro69 of human cardiac Troponin T protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Troponin, working in conjunction with tropomyosin, functions as a molecular switch, regulating muscle contraction in response to changes in the intracellular Ca2+ concentration. Troponin consists of three subunits: the Ca2+-binding subunit troponin C (TnC), the tropomyosin-binding subunit troponin T (TnT), and the inhibitory subunit troponin I (TnI) (1). In response to β-adrenergic stimulation of the heart, Ser23 and Ser24 of TnI (cardiac) are phosphorylated by PKA and PKC. This phosphorylation stimulates a conformational change of the regulatory domain of TnC, reduces the association between TnI and TnC, and decreases myofilament Ca2+ sensitivity by reducing the Ca2+ binding affinity of TnC (1-3).The tropomyosin binding subunit of the troponin complex TnT exists as different isoforms in slow skeletal muscle (ssTnT/TNNT1), fast skeletal muscle (fsTnT/TNNT3) and in cardiac muscle (cTnT/TNNT2). Each of these may also contain multiple alternatively spliced variants. Assays for measuring serum concentrations of cTnT, as well as cTnI, have been reported for analyzing cardiac injury.

1. Ward, D.G. et al. (2002) J. Biol. Chem. 277, 41795-41801.
2. Noland, T.A. et al. (1995) J. Biol. Chem. 270, 25445-25454.
3. Gaponenko, V. et al. (1999) J. Biol. Chem. 274, 16681-16684.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.