Phospho-LCP1 (Tyr28) Antibody

• 产品编号：CST-5277S      品牌：CST       原厂货号：5277S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-LCP1 (Tyr28) Antibody仅检测在Tyr28位点磷酸化的内源性LCP1总蛋白。通过人工合成人源LCP1蛋白Tyr28位点周围相应磷酸化片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。高度保守和广泛表达的plastin蛋白由actin结合蛋白的一部分组成，它包含促进actin捆绑的蛋白。在哺乳动物中发现三种plastins蛋白展示的不同表达水平，包含L-plastin、T-plastin和I-plastin。 T-plastin (plastin-3)蛋白在实体性组织的细胞中被发现，而I-plastin (plastin-1)蛋白在肾脏、结肠和小肠特异性表达(1-3)。L-plastin (plastin-2)或LCP1 (lymphocyte cytosolic protein 1)蛋白主要在造血干细胞和非造血系统肿瘤中表达，包括在结肠癌细胞系中与转移性发展相关的增加性表达。在转移前癌细胞中LCP1蛋白的过表达可诱导入侵和E-cadherin蛋白表达的减少，这是转移性癌细胞的特征(5)。通过T-cell receptor/co-receptor complex (TCR/CD3)与CD2细胞粘附分子或CD28受体相联系的刺激，LCP1蛋白在Ser5位点磷酸化 (6)。在Ser5位点磷酸化增加LCP1蛋白结合F-actin的能力和增加细胞运动性(7,8)。与其它产品使用MS technology一样，对于磷酸化位点的发现Cell Signaling Technology (CST)公司使用PhosphoScan®, CST's LC-MS/MS平台鉴定LCP1蛋白在Tyr28位点磷酸化(9)。LCP1蛋白在Tyr28位点磷酸化在许多白血病细胞系中发现(9-12)。

1. Lin, C.S. et al. (1993) J Biol Chem 268, 2781-92.
2. Lin, C.S. et al. (1994) Mol Cell Biol 14, 2457-67.
3. Delanote, V. et al. (2005) Acta Pharmacol Sin 26, 769-79.
4. Otsuka, M. et al. (2001) Biochem Biophys Res Commun 289, 876-81.
5. Foran, E. et al. (2006) Int J Cancer 118, 2098-104.
6. Wabnitz, G.H. et al. (2007) Eur J Immunol 37, 649-62.
7. Janji, B. et al. (2006) J Cell Sci 119, 1947-60.
8. Klemke, M. et al. (2007) Int J Cancer 120, 2590-9.
9. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
10. Rikova, K. et al. (2007) Cell 131, 1190-203.
11. Gu, T.L. et al. (2007) Blood 110, 323-33.
12. Walters, D.K. et al. (2006) Leuk Res 30, 1097-104.

原厂资料：

Specificity / Sensitivity

Phospho-LCP1 (Tyr28) Antibody detects endogenous levels of LCP1 protein only when phosphorylated on Tyr28.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr28 of human LCP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Highly conserved and widely expressed plastin proteins comprise a subset of actin-binding proteins that include proteins that promote actin bundling. Three plastins exhibiting differential expression are found in mammals and include L-plastin, T-plastin and I-plastin. T-plastin (plastin-3) is found in cells of most solid tissues while I-plastin (plastin-1) is expressed specifically in kidney, colon and small intestine (1-3). L-plastin (plastin-2) or LCP1 (lymphocyte cytosolic protein 1) is mainly expressed in hematopoietic cells and nonhematopoietic tumors, including increased expression correlated with metastatic progression in colon cancer cell lines (4). Overexpression of LCP1 in premetastatic cancer cell lines induces invasion and loss of E-cadherin expression, which is characteristic of metastatic cancer cell lines (5). LCP1 becomes phosphorylated at Ser5 upon stimulation through the T-cell receptor/co-receptor complex (TCR/CD3) in association with the CD2 cell adhesion molecule or the CD28 receptor (6). Phosphorylation at Ser5 increases LCP1 ability to bind F-actin and increase cell motility (7,8).Phosphorylation of LCP1 on Tyr28 was identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's LC-MS/MS platform for phosphorylation site discovery as well as other publications using MS technology (9). Phosphorylation of LCP1 at Tyr28 is seen in many leukemic cell lines (9-12).

1. Lin, C.S. et al. (1993) J Biol Chem 268, 2781-92.
2. Lin, C.S. et al. (1994) Mol Cell Biol 14, 2457-67.
3. Delanote, V. et al. (2005) Acta Pharmacol Sin 26, 769-79.
4. Otsuka, M. et al. (2001) Biochem Biophys Res Commun 289, 876-81.
5. Foran, E. et al. (2006) Int J Cancer 118, 2098-104.
6. Wabnitz, G.H. et al. (2007) Eur J Immunol 37, 649-62.
7. Janji, B. et al. (2006) J Cell Sci 119, 1947-60.
8. Klemke, M. et al. (2007) Int J Cancer 120, 2590-9.
9. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.
10. Rikova, K. et al. (2007) Cell 131, 1190-203.
11. Gu, T.L. et al. (2007) Blood 110, 323-33.
12. Walters, D.K. et al. (2006) Leuk Res 30, 1097-104.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.