Phospho-MERIT40 (Ser29) Antibody

• 产品编号：CST-12110S      品牌：CST       原厂货号：12110S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MERIT40 (Ser29)抗体可以识别内源性29位丝氨酸被磷酸化后的MERIT40蛋白。多抗由合成肽段免疫动物产生，该肽段与人MERIT40蛋白29位丝氨酸邻近残基序列对应。抗体由蛋白A和肽段亲和层析技术纯化得到。乳腺癌易感基因，BRCA1，编码一种E3泛素连接酶，BRCA1通过调控DNA损伤反应和DNA修复维持基因组的稳定性。BRCA1会和其他DNA损伤蛋白结合形成至少三种不同的复合物（BRCA1A,B和C），这种结合对于调控BRCA1的功能是至关重要的。BRCA1-Rap80 复合物 (BRCA1 A复合物)，包括Rap80, BRCC36, BRCC45, Abraxas, 和MERIT40/NBA1,调控G2/M周期检验点（1,2）。MERIT40/NBA1定位于DNA损伤的部位，对于维持电离辐射后引发的BRCA1反应及其正确定位，以及维持BRCA1复合物都是必须的（3.4）。蛋白组学研究发现MERIT40/NBA1的29位丝氨酸可以被磷酸化，该磷酸化的重要性尚待研究（5-9）。

1. Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
2. Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
3. Wang, B. et al. (2009) Genes Dev 23, 729-39.
4. Shao, G. et al. (2009) Genes Dev 23, 740-54.
5. Moritz, A. et al. (2010) Sci Signal 3, ra64.
6. Rigbolt, K.T. et al. (2011) Sci Signal 4, rs3.
7. Iliuk, A.B. et al. (2010) Mol Cell Proteomics 9, 2162-72.
8. Wu, F. et al. (2010) Mol Cell Proteomics 9, 1616-32.
9. Mayya, V. et al. (2009) Sci Signal 2, ra46.

原厂资料：

Specificity / Sensitivity

Phospho-MERIT40 (Ser29) Antibody recognizes endogenous levels of MERIT40 protein only when phosphorylated at Ser29.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser29 of human MERIT40 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The breast cancer susceptibility gene, BRCA1, codes for an E3 ubiquitin ligase that functions in the maintenance of genome stability through regulation of DNA damage response and DNA repair. BRCA1 forms at least three distinct complexes (BRCA1 A, B, and C) with other DNA repair proteins, and these interactions are vital for the regulation of BRCA1 function. The BRCA1-Rap80 complex (BRCA1 A complex), including Rap80, BRCC36, BRCC45, Abraxas, and MERIT40/NBA1, functions in G2/M phase checkpoint control (reviewed in 1,2). 
 MERIT40/NBA1 localizes to sites of DNA damage and is required for the appropriate localization of BRCA1 in response to ionizing radiation, as well as maintenance of the BRCA1 A complex (3,4). Proteomics studies have identified Ser29 as a phosphorylated site on MERIT40/NBA1, and the significance of this phosphorylation is under investigation (5-9).

1. Ohta, T. et al. (2011) FEBS Lett 585, 2836-44.
2. Huen, M.S. et al. (2010) Nat Rev Mol Cell Biol 11, 138-48.
3. Wang, B. et al. (2009) Genes Dev 23, 729-39.
4. Shao, G. et al. (2009) Genes Dev 23, 740-54.
5. Moritz, A. et al. (2010) Sci Signal 3, ra64.
6. Rigbolt, K.T. et al. (2011) Sci Signal 4, rs3.
7. Iliuk, A.B. et al. (2010) Mol Cell Proteomics 9, 2162-72.
8. Wu, F. et al. (2010) Mol Cell Proteomics 9, 1616-32.
9. Mayya, V. et al. (2009) Sci Signal 2, ra46.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.