Phospho-MST1 (Thr183)/MST2 (Thr180) Antibody

• 产品编号：CST-3681S      品牌：CST       原厂货号：3681S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody 仅能够检测分别在threonine 183 和 threonine 180位点磷酸化的内源性的Mst1和Mst2蛋白。该抗体可能与磷酸化的Mst3和Mst4蛋白发生交叉反应。通过人工合成人源Mst1蛋白Thr183位点周围相应的磷酸化肽段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Mst激酶是激酶STE20家族成员，该蛋白是MAPK通路的上游激活因子，调节相关进程例如凋亡、形态形成和细胞骨架重排。研究表明，Mst蛋白的氨基端激酶区域与酵母STE20激酶的激酶结构域及其它p21激活的激酶具有同源性(1)。Mst1和Mst2蛋白的羧基末端区域包含二聚化和抑制区域(1-3)。激活环的二聚化和磷酸化能够导致Mst1蛋白从细胞质迁移到细胞核(3)。不断的研究指出，Mst1、Mst2和Mst3蛋白在凋亡信号和其它应激条件下被激活(4-6)。Mst1蛋白的完全激活需要磷酸化和caspase介导的剪切(4)。GCK家族的激活环的序列比对认为 Mst1 蛋白Thr183位点和 Mst2 蛋白Thr180位点是保守的残基，并且可能对该激酶的活性起着重要作用。活化的 Mst 激酶可能依赖于p38 MAPK和JNK通路去扩大凋亡信号(5)。Mst1蛋白的Ser327位点距离caspase-3识别位点很近，该位点的磷酸化抑制caspase介导的剪切 (4)。

1. Dan, I. et al. (2001) Trends Cell Biol 11, 220-30.
2. Creasy, C.L. et al. (1996) J Biol Chem 271, 21049-53.
3. Lee, K.K. and Yonehara, S. (2002) J Biol Chem 277, 12351-8.
4. Ura, S. et al. (2001) Proc Natl Acad Sci U S A 98, 10148-53.
5. Zhao, B. et al. (2011) Nat Cell Biol 13, 877-83.
6. Deng, Y. et al. (2003) J Biol Chem 278, 11760-7.
7. Praskova, M. et al. (2004) Biochem J 381, 453-62.

原厂资料：

Specificity / Sensitivity

Phospho-Mst1 (Thr183)/Mst2 (Thr180) Antibody detects endogenous Mst1 and Mst2 only when phosphorylated at threonine 183 and threonine 180, respectively. The antibody may cross-react with phosphorylated Mst3 and Mst4.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr183 of human Mst1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Mst kinases, members of the STE20 family of kinases, are upstream activators of MAPK pathways that regulate processes such as apoptosis, morphogenesis and cytoskeletal rearrangements. The amino-terminal kinase domain of Mst is considerably homologous to the kinase domain of yeast STE20 kinase and other p21-activated kinases (1). The carboxy-terminal region of Mst1 and Mst2 contains dimerization and inhibitory domains (1-3). Dimerization and phosphorylation at the activation loop results in translocation of Mst1 from the cytosol to the nucleus (3). Growing evidence indicates that Mst1, Mst2 and Mst3 are activated by apoptotic signals as well as other stress conditions (4-6). Complete activation of Mst1 requires both phosphorylation and caspase-mediated cleavage (4). Sequence alignment of the activation loop of the GCK family indicates that Thr183 of Mst1 and Thr180 of Mst2 are the conserved residues and might be critical for the activity of the kinases. Activated Mst kinases may rely on p38 MAPK and JNK pathways to amplify apoptotic signals (5). Phosphorylation at Ser327 of Mst1, which is close to the caspase-3 recognition site, inhibits caspase-mediated cleavage (4).

1. Dan, I. et al. (2001) Trends Cell Biol 11, 220-30.
2. Creasy, C.L. et al. (1996) J Biol Chem 271, 21049-53.
3. Lee, K.K. and Yonehara, S. (2002) J Biol Chem 277, 12351-8.
4. Ura, S. et al. (2001) Proc Natl Acad Sci U S A 98, 10148-53.
5. Zhao, B. et al. (2011) Nat Cell Biol 13, 877-83.
6. Deng, Y. et al. (2003) J Biol Chem 278, 11760-7.
7. Praskova, M. et al. (2004) Biochem J 381, 453-62.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.