Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody

• 产品编号：CST-3661S      品牌：CST       原厂货号：3661S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody用于检测内源性79位丝氨酸磷酸化的ACC蛋白水平。该抗体同时可识别ACCalpha和ACCbeta。多克隆抗体通过用对应于人源ACC蛋白Ser79序列的合成肽免疫动物制备而得，抗体经蛋白A和肽段亲和层析纯化制得。乙酰辅酶A合成酶(ACC)用于催化脂肪酸合成途径中的关键步骤。分子量为265 kDa的ACCα，是肝脏，脂肪细胞和乳腺中ACC的主要形式，而在骨胳肌和心脏中，其主要形式为280 kDa的ACCβ(1)。ACC可以在Ser79被AMPK磷酸化，或者在Ser1200被PKA磷酸化，这些位点的磷酸化可以抑制ACC的酶活性(2)。ACC也是减肥药物的一个潜在靶点(3,4)。

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.
6. Hadad, S.M. et al. (2009) BMC Cancer 9, 307.
7. Fullerton, M.D. et al. (2013) Nat Med 19, 1649-54.

原厂资料：

Specificity / Sensitivity

Phospho-Acetyl-CoA Carboxylase (Ser79) Antibody detects endogenous levels of ACC only when phosphorylated at serine 79. The antibody recognizes both ACCalpha and ACCbeta.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser79 of rat ACC. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Acetyl-CoA Carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.
6. Hadad, S.M. et al. (2009) BMC Cancer 9, 307.
7. Fullerton, M.D. et al. (2013) Nat Med 19, 1649-54.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.