WAVE-2 (D2C8) XP? Rabbit mAb

• 产品编号：CST-3659S      品牌：CST       原厂货号：3659S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

WAVE-2 (D2C8) XP® Rabbit mAb检测内源性WAVE-2总蛋白。通过人工合成人源WAVE-2蛋白相应的多肽片段去免疫动物从而制备出单克隆抗体.Wiskott-Aldrich syndrome proteins (WASPs)在活化的Rho家族GTPases反应下通过激活Arp2/3肌动蛋白成核复合物从而介导肌动蛋白的动力。在哺乳动物中，五个WASP家族成员已经被描述。造血细胞中的WASP和广泛表达的N-WASP蛋白在未刺激的细胞中被自抑制。在刺激情况下它们通过cdc42激酶被激活，这与磷酯酰二磷酸肌醇一起解除了自抑制。三个WAVE(Wasf, SCAR)家族蛋白在与WASP和N-WASP序列上有类似性，但是缺乏WASP/N-WASP蛋白的自抑制区域以及直接被Rac激酶激活(1)。WASP和WAVE蛋白的功能显得至关重要，因为在小鼠中N-WASP或Scar-2基因敲除导致心脏和神经元的损失和胚胎的致敏性(2,3)。WASP的缺少导致免疫系统的缺损和减少免疫细胞(4)。WAVE-2 (WASF2)是广泛的分布，而WAVE-1和WAVE-3是在大脑中高表达(5)。WAVE-3蛋白可能作为一个成神经细胞瘤的肿瘤抑制因子，该病是一种交感神经系统的儿童疾病(6)。WAVE-3蛋白的增加性表达在乳腺癌中被发现，并且在乳腺癌细胞中的研究指出WAVE-3蛋白通过p38 mitogen-activated protein kinase (MAPK)通路去调节乳腺癌的进程、入侵和代谢(7,8)。

1. Millard, T.H. et al. (2004) Biochem J. 380, 1-17.
2. Yan, C. et al. (2003) EMBO J. 22, 3602-3612.
3. Snapper, S.B. et al. (2001) Nat. Cell Biol. 3, 897-904.
4. Zhang, J. et al. (1999) J. Exp. Med. 190, 1329-4132.
5. Suetsugu, S. et al. (1999) Biochem. Biophys. Res. Commun. 260, 296-302.
6. Sossey-Alaoui, K. et al. (2002) Oncogene 21, 5967-5974.
7. Sossey-Alaoui, K. et al. (2005) Exp. Cell Res. 308, 135-145.
8. Sossey-Alaoui, K. et al. (2007) Am J Pathol 170, 2112-21.

原厂资料：

Specificity / Sensitivity

WAVE-2 (D2C8) XP® Rabbit mAb detects endogenous levels of total WAVE-2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human WAVE-2.

Background

Wiskott-Aldrich syndrome proteins (WASPs) mediate actin dynamics by activating the Arp2/3 actin nucleation complex in response to activated Rho family GTPases. In mammals, five WASP family members have been described. Hematopoietic WASP and ubiquitously expressed N-WASP are autoinhibited in unstimulated cells. Upon stimulation they are activated by cdc42, which relieves the autoinhibition in conjunction with phosphatidyl inositol 4,5-bisphosphate. Three WAVE (Wasf, SCAR) family proteins are similar in sequence to WASP and N-WASP but lack the WASP/N-WASP autoinhibition domains and are indirectly activated by Rac (reviewed in 1). Both WASP and WAVE functions appear to be essential, as knockout of either N-WASP or Scar-2 in mice results in cardiac and neuronal defects and embryonic lethality (2,3). Loss of WASP results in immune system defects and fewer immune cells (4). WAVE-2 (WASF2) is widely distributed, while WAVE-1 and WAVE-3 are strongly expressed in brain (5). WAVE-3 may act as a tumor suppressor in neuroblastoma, a childhood disease of the sympathetic nervous system (6). Increased expression of WAVE-3 is seen in breast cancer, and studies in breast adenocarcinoma cells indicate that WAVE-3 regulates breast cancer progression, invasion and metastasis through the p38 mitogen-activated protein kinase (MAPK) pathway (7,8).

1. Millard, T.H. et al. (2004) Biochem J. 380, 1-17.
2. Yan, C. et al. (2003) EMBO J. 22, 3602-3612.
3. Snapper, S.B. et al. (2001) Nat. Cell Biol. 3, 897-904.
4. Zhang, J. et al. (1999) J. Exp. Med. 190, 1329-4132.
5. Suetsugu, S. et al. (1999) Biochem. Biophys. Res. Commun. 260, 296-302.
6. Sossey-Alaoui, K. et al. (2002) Oncogene 21, 5967-5974.
7. Sossey-Alaoui, K. et al. (2005) Exp. Cell Res. 308, 135-145.
8. Sossey-Alaoui, K. et al. (2007) Am J Pathol 170, 2112-21.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.