Phospho-Ras-GRF1 (Ser916) Antibody

• 产品编号：CST-3321S      品牌：CST       原厂货号：3321S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Ras-GRF1 (Ser916) Antibody 兔多抗识别转染水平的Ser916磷酸化的Ras-GRF1蛋白。此抗体不与磷酸化的Ras-GRF2蛋白交叉反应。该多克隆抗体由合成的小鼠Ras-GRF1蛋白Ser916位点附近磷酸化肽段免疫动物而制成。该抗体使用蛋白A和多肽亲和层析纯化而得。Ras活性被GAP（GTP酶激活蛋白）和GEFs（鸟嘌呤核苷酸交换因子）调控。RAS-GRF1（也称为CDC25Mm）是神经RasGEF，被异三聚体G蛋白和钙离子内流所调节（1,2）。结合钙调蛋白和磷酸化可以激活Ras-GRF1的活性（1,2）。多个PKA磷酸化位点已在Ras-GRF上被确定。Ser54和Ser822是磷酸化的两个主要位点，抑制RAS-GRF的活性（3）。Carbachol（毒蕈碱激动剂）引起的Ser916磷酸化是Ras-GRF活性必要但不充分的条件（4）。据报道，RAS-GRF1被酪氨酸激酶Src磷酸化后，也显示出GEF对Rac的活性（5）。

1. Mattingly, R.R. and Macara, I.G. (1996) Nature 382, 268-72.
2. Farnsworth, C.L. et al. (1995) Nature 376, 524-7.
3. Baouz, S. et al. (2001) J. Biol. Chem. 276, 1742-1749.
4. Mattingly, R.R. (1999) J Biol Chem 274, 37379-84.
5. Kiyono, M. et al. (2000) J. Biol. Chem. 275, 29788-29793.

原厂资料：

Specificity / Sensitivity

Phospho-Ras-GRF1 (Ser916) Antibody detects transfected levels of Ras-GRF1 only when phosphorylated at serine 916. This antibody does not cross-react with phosphorylated Ras-GRF2.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serine 916 of mouse Ras-GRF1. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ras activity is regulated by GAP (GTPase activating proteins) and GEFs (guanine nucleotide exchange factors). Ras-GRF1 (also known as CDC25Mm) is neuronal RasGEF and is regulated by heterotrimeric G proteins and calcium influx (1,2). Binding to calmodulin and phosphorylation stimulate Ras-GRF1 activity (1,2). Multiple PKA phosphorylation sites on Ras-GRF have been identified. Phosphorylation on the two major sites, Ser54 and Ser822, inhibits Ras-GRF activity (3). Carbachol (a muscarinic agonist)-induced phosphorylation on Ser916 is essential but not sufficient for maximal Ras-GRF activity (4). It has been reported that Ras-GRF1 also shows GEF activity toward Rac after phosphorylation by the tyrosine kinase Src (5).

1. Mattingly, R.R. and Macara, I.G. (1996) Nature 382, 268-72.
2. Farnsworth, C.L. et al. (1995) Nature 376, 524-7.
3. Baouz, S. et al. (2001) J. Biol. Chem. 276, 1742-1749.
4. Mattingly, R.R. (1999) J Biol Chem 274, 37379-84.
5. Kiyono, M. et al. (2000) J. Biol. Chem. 275, 29788-29793.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.