Phospho-C/EBPβ (Thr235) Antibody

• 产品编号：CST-3084S      品牌：CST       原厂货号：3084S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-C/EBPβ(Thr235)抗体仅识别内源性的Thr235磷酸化的人LAP蛋白水平，Thr188磷酸化的小鼠和大鼠LAP蛋白和Thr37磷酸化的LIP蛋白。此抗体与其它磷酸化的C/EBP亚型没有交叉反应。该多克隆抗体用与人C/EBPβ中Thr235位点附近的氨基酸序列对应的人工合成磷酸化的肽段免疫动物制成。该抗体使用蛋白A和肽亲和层析纯化而得。CCAAT/增强子结合蛋白（C/EBP）是一个转录因子家族，对细胞分化、终端功能和炎症反应十分关键（1）。目前已确定该家族的6个成员（C/EBPα, β, δ, γ, ε, ζ），它们分布在不同组织（1）。C/EBPβ有两种形式，38kDa的肝激活蛋白（LAP）和20kDa的肝抑制蛋白（LIP），它们可能是可变翻译的产物。38kDa LAP蛋白是转录激活因子，LIP是C/EBPβ的转录活性的抑制剂（2）。C/EBPβ不同位点的磷酸化刺激其转录活性（3-5）。大鼠C/EBPβ Ser105磷酸化是仅在大鼠序列中出现的一个独特位点，似乎对大鼠C/EBPβ的激活至关重要（6）。

1. Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
2. Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920-1932.
3. Wegner, M. et al. (1992) Science 256, 370-373.
4. Trautwein, C. et al. (1993) Nature 364, 544-547.
5. Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207-2211.
6. Buck, M. et al. (1999) Mol. Cell 4, 1087-1092.

原厂资料：

Specificity / Sensitivity

Phospho-C/EBPbeta (Thr235) Antibody detects endogenous levels of human LAP only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and LIP only when phosphorylated at Thr37. This antibody does not cross-react with other phosphorylated C/EBPs.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding threonine 235 of human C/EBPbeta. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPα, -β, -γ, -δ, -ε and -ζ) and are distributed in a variety of tissues (1). There are two forms of C/EBPβ, the 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP) which may be products of alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPβ transcriptional activity (2). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at serine 105 of rat C/EBPβ, a unique site only present in the rat sequence, seems essential for rat C/EBPβ activation (6).

1. Lekstrom-Himes, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
2. Calkhoven, C.F. et al. (2000) Genes Dev. 14, 1920-1932.
3. Wegner, M. et al. (1992) Science 256, 370-373.
4. Trautwein, C. et al. (1993) Nature 364, 544-547.
5. Nakajima, T. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 2207-2211.
6. Buck, M. et al. (1999) Mol. Cell 4, 1087-1092.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.