E2A (D2B1) Rabbit mAb

• 产品编号：CST-12258S      品牌：CST       原厂货号：12258S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

E2A (D2B1) Rabbit mAb兔单抗能够检测内源性E2A总蛋白水平。此单克隆抗体通过合成肽免疫动物制备，这种合成肽为人源E2A蛋白Gly112周围肽段。E2A是转录因子E-蛋白家族的成员, 是一种碱性螺旋-环-螺旋(bHLH)蛋白亚类，能够特异性的结合到E-box共有性序列上(1,2)。可变剪切产生了两个E2A 亚型(E47 和 E12)，它们活跃的参与到B细胞命运决定、B淋巴细胞成熟、IgK V-J重组、外周血B细胞的发育与肿瘤的抑制过程中(3)。E2A在G1期免疫球蛋白多样化分化过程中发挥顺式调节作用(4)。E2A基因染色体的易位导致了多种融合蛋白的表达并与小儿急性淋巴细胞性白血病有关(5)。

1. Murre, C. (2005) Nat Immunol 6, 1079-86.
2. Bain, G. et al. (2001) Nat Immunol 2, 165-71.
3. Schwartz, R. et al. (2006) Proc Natl Acad Sci U S A 103, 9976-81.
4. Yabuki, M. et al. (2009) J Immunol 182, 408-15.
5. LeBrun, D.P. (2003) Front Biosci 8, s206-22.

原厂资料：

Specificity / Sensitivity

E2A (D2B1) Rabbit mAb recognizes endogenous levels of total E2A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly112 of human E2A protein.

Background

E2A is a member of the E-protein family of transcription factors, a subclass of basic helix-loop-helix (bHLH) proteins that bind specifically to E-box consensus sequences (1,2). Alternative splicing generates two E2A isoforms (E47 and E12) that are actively involved in B cell lineage commitment, B cell maturation, IgK V-J rearrangement, peripheral B cell development, and tumor suppression (3). E2A acts in cis during G1 to promote immunoglobulin gene diversification (4). Research studies have shown that chromosomal translocations involving the E2A gene result in the expression of multiple fusion proteins and are associated with many cases of pediatric acute lymphoblastic leukemia (5).

1. Murre, C. (2005) Nat Immunol 6, 1079-86.
2. Bain, G. et al. (2001) Nat Immunol 2, 165-71.
3. Schwartz, R. et al. (2006) Proc Natl Acad Sci U S A 103, 9976-81.
4. Yabuki, M. et al. (2009) J Immunol 182, 408-15.
5. LeBrun, D.P. (2003) Front Biosci 8, s206-22.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.