ATPases that belong to the ATPase associated with different cellular activities (AAA) family are homo-oligomeric proteins that have roles in
cell cycle regulation, protein degradation, organelle biogenesis, and vesicle mediated-protein transport. Valosin containing protein (VCP) is a
AAA family member that is found as a hexamer in rat liver. In vitro, VCP catalyzes the hydrolysis of ATP, but this activity is distinct from
classical transport ATPases. VCP protein is found primarily in the transitional elements between the rough and smooth ER (TER), but
stimulation with EGF leads to VCP translocation to the nucleus. Antibodies to VCP can perturb cell-free formation of transition vesicles from
isolated TER of rat liver, and can inhibit transfer of material from ER to Golgi in a reconstituted membrane system. VCP is phosphorylated
after T-cell activation, and PTPH1 dephosphorylation of VCP correlates with suppression of cell growth. In addition, the N-terminal region of
VCP binds to the DNA damage repair protein, BRCA1. Thus, VCP may have roles in both ER protein transport and nuclear function, which
are important for cell growth and survival.
原厂资料：
注意事项：
1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
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