Hu/NHP CD28 FITC CD28.2 50Tst

The CD28.2 monoclonal antibody specifically binds to CD28, a 44 kDa homodimeric transmembrane glycoprotein present on most mature T 

cells, thymocytes and plasma cells. CD28 is a costimulatory receptor that binds CD80 and CD86 as ligands and plays a very important role in 

T cell-B cell interactions. It has been suggested that CD28 initiates and regulates a separate and distinct signal transduction pathway from 

those stimulated by the TCR complex. Additionally, it has been reported that CD28 antibody clones vary in their ability to stimulate T cells to 

produce IL-2 and increase intracellular Ca2+ concentration. This finding suggests the existence of functionally distinct subregions on the 

CD28 molecule. CD28.2 has been demonstrated to bind to the same molecule as clone L293, another CD28 mAb, and has been reported to 

induce Ca2+ influx in Jurkat T cells.

This clone also cross-reacts with a subset of peripheral blood lymphocytes, but not monocytes or granulocytes, of baboon, rhesus and 

cynomolgus macaques. The distribution on lymphocytes is similar to that observed with normal human donor lymphocytes; however, as 

described in the literature, the frequency of CD28.2-positive lymphocytes is reduced in the non-human primate, with the majority of cells 

being CD28 negative.

 

 

1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 X 10e6 cells in a 100-μl experimental sample (a test).

2.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

3.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

4.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

5.The antibody was conjugated with FITC under optimum conditions, and unconjugated antibody and free FITC were removed.