T4 DNA Ligase Reaction Buffer

产品编号：B0202S 品牌：New England Biolabs 原厂货号：B0202S
产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
应用分类：

包装：

运保温度:

-20°C

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描述：

Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.

Reagents Supplied:
10X T4 DNA Ligase Reaction Buffer

1X T4 DNA Ligase Reaction Buffer:
50 mM Tris-HCl
10 mM MgCl₂
10 mM Dithiothreitol
1 mM ATP
pH 7.5 @ 25°C

Quality Control for Current Lot

16-Hour Incubation::
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity::
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.

注意事项：

注意事项：使用前，反应缓冲液 (NEBuffer) 应彻底融化并混匀。