Taq DNA Ligase Reaction Buffer

产品编号：B0208S 品牌：New England Biolabs 原厂货号：B0208S
产品分类：生化试剂 > 生物缓冲液 > 分子生物酶缓冲液
应用分类：

包装：

运保温度:

-20°C For long term storage (>30 days), store at -

到货周期：

登录后查看

标准价：

￥客户可见

会员价：

￥客户可见

积分：

客户可见分

登录之后可加入购物车购买，请您

运费与支付说明：

1.含干冰类产品有运费；

2. 必须现金付款或有信用额度的会员才可以直接发货，否则需要等待现金付款信息。

描述：

Description:
New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.

Reagents Supplied:
10X Taq DNA Ligase Reaction Buffer
λ DNA-BstEII Digest

1X Taq DNA Ligase Reaction Buffer:
20 mM Tris-HCl
25 mM potassium acetate
10 mM Magnesium Acetate
10 mM Dithiothreitol
1 mM NAD
0.1 % Triton X-100
pH 7.6 @ 25°C

Quality Control for Current Lot

Quality control values for a specific lot can be found on the datacard which accompanies each product.

16-Hour Incubation::
A 50 µl reaction containing this reaction buffer at a 1X concentration and 1 µg of HaeIII digested ΦX174 RF I DNA incubated for 16 hours resulted in no detectable non-specific nuclease degradation.

Endonuclease Activity::
Incubation of this reaction buffer at a 1X concentration with 1 µg ΦX174 RF I DNA for 4 hours at 37°C in 50 µl reactions resulted in less than 5% conversion to RF II.

注意事项：

注意事项：使用前，反应缓冲液（NEBuffers）应彻底融化并混匀。