TaqMan® Mutation Detection Assays detect somatic mutations in genes that are associated with cancer. These research use only assays are powered by competitive allele-specific TaqMan® PCR technology, also known as castPCR™ technology. The castPCR™ technology is highly specific and sensitive, and it can detect rare amounts of mutated DNA in a sample that contains large amounts of normal, wild type DNA.
High specificity—mutant allele detection is based on an allele-specific primer, while the wild-type background is suppressed by an MGB blocker oligonucleotide
High sensitivity—assays can detect as low as 0.1% mutant molecules in a background of wild type gDNA as demonstrated in spiking experiments
Wide dynamic range and excellent PCR efficiency—assays demonstrate at least 4 logs of dynamic range and an average efficiency of 100 ± 10%
Fast, simple workflow—like other TaqMan® Assays, typically requires 3 hours from sample to results, with minimum hands-on time
Our current assay set covers 778 assays to key mutation targets in 46 cancer genes
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For Research Use Only. Not for use in diagnostic procedures.