Thermo Scientific DyNAmo ColorFlash SYBR Green qPCR Kit offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.
Highlights
•Colored reagents minimize the risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
•Extremely fast protocols due to combined annealing and extension step of only 15 s
•Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2x master mix allows the use of UNG for prevention of carry-over contamination
Applications
• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye
Includes
• 2X master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, MgCl2, dNTP mix including dUTP)
• 40X sample buffer solution with yellow dye
• 50X ROX passive reference dye
Shipping and storage
DyNAmo ColorFlash qPCR Kit is shipped on gel ice. Upon arrival, store all kit components at -20°C. When using the 2X master mix, the leftover thawed mix can be refrozen and stored at -20°C without affecting the performance of the kit.
The yellow sample buffer solution is stable and can be stored at +4°C, but storage at -20°C with the other kit components is recommended.
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