Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM ⁄ F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM ⁄ F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. Life Technologies offers a variety of Gibco® DMEM ⁄ F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM ⁄ F-12 is modified as follows:
With
Without
• GlutaMAX™
• HEPES
• Phenol Red
The complete formulation is available.
Gibco® DMEM ⁄ F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids and vitamins with F-12’查看更多s wide variety of components. Gibco® DMEM ⁄ F-12 with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.
Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System
Gibco® DMEM ⁄ F-12 is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards. For supply chain continuity, Life Technologies offers an identical Gibco® DMEM ⁄ F-12 product made in our Scotland facility (31331-093). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
DMEM ⁄ F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM ⁄ F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM ⁄ F-12 uses a sodium bicarbonate buffer system (2.438 g ⁄ L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
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