BRM Antibody

• 产品编号：CST-6889S      品牌：CST       原厂货号：6889S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

BRM Antibody能够检测内源性BRM总蛋白水平。该抗体不与BRG1蛋白发生交叉反应。通过合成的与人源BRM蛋白Gly264位点周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。ATP依赖的染色质重塑复合物在调节多种细胞核进程中扮演重要作用，例如基因表达、DNA复制和修复(1, 2)。SWI/SNF染色质重塑复合物是由10个亚单位和ATPase催化亚单位BRM或BRG1其中一个单独分子组成。这些两种亚单位的激活可干扰组蛋白和DNA的联系，这导致改变了染色质中重要的调节因子元件的易接近性(2-5)。通过转录因子例如细胞核受体、p53、RB和BRCA1，含有SWI/SNF的BRM/BRG1复合物被招募到靶启动子上去调节基因激活、细胞生长、细胞周期和分化过程(1, 6-9)。BRM和BRG1也被考虑是肿瘤抑制因子，以及它们的表达水平在数种癌症细胞系中急剧减少(10-13)。

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Yamamichi, N. et al. (2005) Oncogene 24, 5471-81.
11. Reisman, D.N. et al. (2002) Oncogene 21, 1196-207.
12. Shen, H. et al. (2008) Cancer Res 68, 10154-62.
13. Weissman, B. and Knudsen, K.E. (2009) Cancer Res 69, 8223-30.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

BRM Antibody recognizes endogenous levels of total BRM protein. This antibody does not cross-react with BRG1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly264 of human BRM protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

ATP-dependent chromatin remodeling complexes play an essential role in the regulation of various nuclear processes such as gene expression, DNA replication, and repair (1, 2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits with a single molecule of the ATPase catalytic subunit BRM or BRG1, but not both. The activities of these two subunits drive the disruption of histone-DNA contacts that lead to changes in accessibility of crucial regulatory elements within chromatin (2-5). The BRM/BRG1 containing SWI/SNF complexes are recruited to target promoters by transcription factors such as nuclear receptors, p53, RB, and BRCA1 to regulate gene activation, cell growth, the cell cycle, and differentiation processes (1, 6-9). BRM and BRG1 are also considered to be tumor suppressors and their expression levels are severely reduced in several cancer cell lines (10-13).

1. Ho, L. and Crabtree, G.R. (2010) Nature 463, 474-84.
2. Becker, P.B. and Hörz, W. (2002) Annu Rev Biochem 71, 247-73.
3. Eberharter, A. and Becker, P.B. (2004) J Cell Sci 117, 3707-11.
4. Bowman, G.D. (2010) Curr Opin Struct Biol 20, 73-81.
5. Gangaraju, V.K. and Bartholomew, B. (2007) Mutat Res 618, 3-17.
6. Lessard, J.A. and Crabtree, G.R. (2010) Annu Rev Cell Dev Biol 26, 503-32.
7. Morettini, S. et al. (2008) Front Biosci 13, 5522-32.
8. Wolf, I.M. et al. (2008) J Cell Biochem 104, 1580-6.
9. Simone, C. (2006) J Cell Physiol 207, 309-14.
10. Yamamichi, N. et al. (2005) Oncogene 24, 5471-81.
11. Reisman, D.N. et al. (2002) Oncogene 21, 1196-207.
12. Shen, H. et al. (2008) Cancer Res 68, 10154-62.
13. Weissman, B. and Knudsen, K.E. (2009) Cancer Res 69, 8223-30.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.