LIBRARY EFF DH5@ COMP CELLS 1 ML (5 X 0.2ML) #18263

• 产品编号：18263012      品牌：invitrogen       原厂货号：18263012
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 细菌菌株和感受态细胞 > 感受态细胞
• 应用分类：克隆及合成生物学 > 转化 > 化学感受态细胞

描述：

Library Efficiency® DH5α™ Competent Cells are a versatile strain of chemically competent cells that demonstrate transformation efficiencies of >1 x 108 cfu/µg plasmid DNA. Library Efficiency® DH5α™ Competent Cells are ideal for difficult cloning constructions or any application that uses limiting amounts of DNA. Features of these cells:

• Supports blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Maintains plasmid DNA with minimum recombination events
• Delivers high-yield plasmid preparations for downstream applications
• M13mp cloning vectors may be propagated on a lawn of DH5α-FT™, DH5αF'™, DH5αF'IQ™, JM101, or JM107 for plaque formation

Ideal for difficult cloning projects
Library Efficiency® DH5α™ Competent Cells are recommended for routine cloning of genes into large plasmids, and are suitable for difficult clone constructions, such as blunt-end ligations. Library Efficiency® DH5α™ Competent Cells have a mid-range transformation efficiency (>1 x 108 cfu/µg control DNA per 100 µL reaction). The strain also has the Φ80lacZΔM15 genotype, allowing blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the recA1 mutation helps reduce the rate of recombination while propagating plasmid DNA, and the endA1 mutation makes DH10B™ cells an excellent choice for amplifying plasmid DNA for subsequent extraction and purification.

Genotype: F- Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44thi-1 gyrA96 relA1 λ-

原厂资料：

注意事项：

For Research Use Only. Not for use in diagnostic procedures.