T4 RNA LIGASE 2500 U (5 U/UL) EACH

产品编号：AM2141 品牌：invitrogen 原厂货号：AM2141
产品分类：克隆 > 限制性内切酶和连接酶 > 连接酶
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运保温度:

–20°C

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描述：

Ambion® T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oligonucleotides. One tube containing 500 U (5 U⁄μL) is provided along with 10X Reaction Buffer. The enzyme can be used for tagging the 5' ends of mRNA with oligonucleotides for mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules.

Ambion® T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oligonucleotides. One tube containing 500 U (5 U⁄μL) is provided along with 10X Reaction Buffer. The enzyme can be used for tagging the 5' ends of mRNA with oligonucleotides for mapping studies (RACE), 3'-end labeling RNA molecules, and circularizing RNA and DNA molecules.

Unit Definition:
One unit catalyzes the formation of 1 nmol of [5'-³²P]-rA_12-18 into a phosphatase-resistant form in 30 min at 37°C.One unit catalyzes the formation of 1 nmol of [5'-³²P]-rA_12-18 into a phosphatase-resistant form in 30 min at 37°C.