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PCR2.1 VECTOR 20 REACTIONS

 
包装:
运保温度:
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1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

其它组分:

pCR®2.1, linearized【包装:5 x 10 μl,,运保温度:-20ºC】
10X PCR Buffer【包装:100 μl,,运保温度:-20ºC】
10X Ligation Buffer【包装:100 μl,,运保温度:-20ºC】
50 mM dNTPs【包装:10 μl,,运保温度:-20ºC】
T4 DNA Ligase【包装:25 μl,,运保温度:-20ºC】
Sterile Water【包装:1 ml,,运保温度:-20ºC】
Control DNA Template【包装:10 μl,,运保温度:-20ºC】
Control PCR Primers【包装:10 μl,,运保温度:-20ºC】


描述:

TA Cloning® Kits are designed for cloning Taq-amplified PCR products directly from a PCR reaction using an overnight ligation step. The kits use a pCR® vector and yield >=80% recombinants with >=90% of the recombinants containing inserts. Some of the convenient features of the pCR® vectors are:

  • 3´-T overhangs for direct ligation of Taq-amplified PCR products
  • Choice of T7 (pCR®2.1) or T7 and Sp6 (pCR®II) promoters for in vitro RNA transcription and sequencing
  • Versatile polylinker with flanking EcoR I sites for easy excision of inserts
  • Choice of kanamycin or ampicillin selection
  • Easy blue/white colony screening
  • M13 forward and reverse primer sites for sequencing


注意事项:

TA Cloning® Kits contain linearized pCR®2.1 or pCR®II vector, T4 DNA ligase, 10X ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot® Chemically Competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot® E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.


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