PFHM-II is a protein-free, ready-to-use medium that contains no polypeptide growth or attachment factors, or mediators that may complicate downstream processing and final product purification (1). PFHM-II performs as well as serum-supplemented media for MAb production and also may be used as a growth medium.
In instances where antibiotics are required, most general antibiotics are compatible with PFHM-II, including penicillin/streptomycin, gentamicin, anti-PPLO, linocin, and Fungizone™ Reagent. Do not use kanamycin sulfates or neomycin sulfates.
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