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SPE I 200U #85514

 
包装:
运保温度: Store enzyme and buffer at -20°C
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描述:

5´-A↓CTAGT-3´ 3´-TGATC↑A-5´

Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical services department immediately if you have questions or concerns.

Concentration 10 U/ µL
Contents Spe I 10X Buffer M (1mL) 10X Loading Buffer (1mL)
Reaction Temperature 37°C
Reaction Mixture Spe I 1 µL 10X Buffer M 2 µL Substrate DNA <1 µg Sterile water to 20 µL
Heat inactivation Enzyme is inactivated by heating at 80°C for 20 minutes.
Star Activity Unrelated site may be cut at low ionic strength.
Effect of DNA Methylation Enzyme activity is unaffected by DNA methylation.
Unit Definition One unit is the amount of enzyme required to completely digest 1 µg of λDNA in 50 µL of the reaction mixture in one hour at 37°C.
10X Buffer M 100 mM Tris-HCl, pH 7.5 100 mM MgCl2 10 mM Dithiothreitol 500 mM NaCl
Source Sphaerotilus natans

Relative Activity in Universal Reaction Buffers

 

Buffer L M H K T(+BSA)
Relative Activity (%) 80* 100 80 100 80*
*Weak star activity is detected.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.


注意事项:

For Research Use Only. Not for use in diagnostic procedures.


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