The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ~645/660). Although the substrate itself is fluorescent (excitation/emission maxima ~460/610 nm), the difference between the substrate's and the hydrolysis product is over 200 nm, along the two species to be easily distinguished.
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For Research Use Only. Not for use in diagnostic procedures.