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描述:
Details
Tested species reactivity
Rabbit
Published species reactivity
Not Applicable
Host / Isotype
Goat / IgG
Class
Polyclonal
Type
Secondary Antibody
Immunogen
Gamma Immunoglobin
Conjugate
Alexa Fluor® 488-10 nm colloidal gold
Form
liquid
Concentration
2 mg/ml
Purification
purified
Storage buffer
PBS, pH 7.5
Contains
5mM sodium azide
Storage Conditions
4° C, store in dark
Target Class
IgG
Antibody Form
Whole Antibody
Tested Applications
Dilution *
Immunomicroscopy (IM)
0.2-10 µg/mL
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This 10 nm colloidal gold conjugate may be used as a probe in immunoblotting, light microscopy, fluorescence microscopy or electron microscopy. The fluorescence of this conjugate can be easily detected by standard techniques, but the ease of visualization of colloidal gold can be greatly improved using silver-enhancement methods, such as those we provide in the LI Silver Enhancement Kit (Cat. no. L-24919).
Background/Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
原厂资料:
注意事项:
For Research Use Only. Not for use in diagnostic procedures.