The Alexa Fluor® 488 labeled anti-fluorescein/Oregon Green® goat IgG antibody can be used to enhance the green-fluorescent signal of the fluorescein or Oregon Green® hapten with the superior photostability of the Alexa Fluor® 488 dye while utilizing existing fluorescein-labeled probes and fluorescein-compatible optics. This strategy is utilized in the Alexa Fluor® 488 Signal Amplification Kit for Fluorescein-Conjugated Probes (A11053). This antibody also enables the use of fluorescein or Oregon Green® dyes as haptens instead of biotin. Unlike biotin, which is an endogenous ligand in mitochondria, dye-based haptens permit background-free staining of cells and tissues and a sensitive alternative to biotin-streptavidin methods for ELISAs
原厂资料:
Anti-fluorescent dye antibodies recognize specific fluorophores and, in most cases, quench their fluorescence. Thus many anti-dye antibodies, including those that recognize fluorescein, can serve as cell-impermeant probes for determining whether fluorescent dye-conjugated ligands, proteins, bacteria or other biomolecules have been internalized by endocytic or pinocytic processes.
注意事项:
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.