This antibody can be used to detect expression of C-terminal His fusion proteins from bacterial, insect, and mammalian cells.
This product contains enough material for 25 Western blots. It is functionally tested using 500ng of an E. coli expressed fusion protein containing a C-terminal polyhistidine (6xHis) epitope. A signal can be detected when tested against 100ng of recombinant Positope™ protein. For Western blot, dilute in PBS containing 0.05% Tween-20 and 5% nonfat, dry milk (PBSTM).
This product has also been used successfully to immunoprecipitate fusion proteins that contain the 6x His tag.
Using chemiluminescence as the detection method, no cross-reactivity has been observed in bacterial lysates. In mammalian lysates, a few cross-reactive proteins have been observed upon overexposure of blots.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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