This product contains enough material for 25 Western blots. Western blot experiments with 100ng of recombinant Positope™ control protein gave a detectable signal within 15 minutes using a chromogenic substrate. The Positope™ control protein is a 53 kDa recombinant protein that contains seven epitope tags, including His(C-term), HisG, c-myc, and V5. For Western blot, we recommend diluting the AP-conjugated antibody in Tris-Buffered Saline (TBS) containing 0.1% (v/v) Tween-20 and 1% (w/v) nonfat dry milk.
In Western blot applications, no cross-reactivity has been observed in bacterial lysates.
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注意事项：
For Research Use Only. Not for use in diagnostic procedures.
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