Thermo Scientific Phi6 RNA Replicase is a modified version of the protein P2 from bacteriophage Phi6. This novel RNA-dependent RNA polymerase catalyzes the synthesis of a full-length complementary RNA strand initiating from the 3’ terminus of a single-stranded RNA. The polymerase does not require any oligonucleotide primer for the initiation. Due to a unique modification, the Phi6 RNA Replicase displays relatively low template specificity, and it is therefore capable of replicating a great variety of RNA templates as well as denatured DNA that contains a recognition sequence for the Phi6 RNA Replicase at its 3’ terminus.
Applications
• Replication of ssRNA to dsRNA form
• Synthesis and amplification of dsRNA using dsDNA as template
• For producing larger amounts of dsRNA, the Replicator RNAi Kit is recommended
Includes
• Phi6 RNA Replicase is supplied with 10X Phi6 RNA Replicase Buffer and 50 mM MnCl2 solution. 1X buffer contains: 50 mM Tris-HCl (pH 8.75 at 21°C), 50 mM NH4Ac.