PowerCut Dicer

• 产品编号：F-602L      品牌：Finnzymes       原厂货号：F-602L
• 产品分类：Discontinued已停产
• 应用分类：

描述：

The novel Thermo Scientific PowerCut Dicer produces a pool of 25 to 27 nucleotide-long siRNA molecules from any dsRNA. This efficient endoribonuclease originating from Giardia intestinalis outperforms all other siRNA producing enzymes by cleaving the substrate with 100% efficiency, yet leaving the resulting siRNA intact. For example, the high quality siRNA produced is ideal for gene silencing studies. Pooled siRNAs are also preferred over synthetic siRNA oligos if the target sequence cannot be specified well enough or when the sequence is prone to variations like in the case of viral targets.

PowerCut Dicer combined with the Replicator RNAi Kit is a powerful combination for generating high amounts of siRNA from in vitro-produced dsRNA.

Highlights
100 % cleavage efficiency gives maximal siRNA yields
No overdigestion of siRNAs
Produces siRNAs of 25 to 27 nucleotide-long siRNAs with a strong silencing function
Free of contaminating RNase, endo-, and exonuclease activities

Includes
PowerCut Dicer is supplied with 5x PowerCut Dicer Reaction Buffer

siRNAs with 100% efficiency without overdigestion
Three different dsRNA substrates (Furin, eGFP, and Phi6 dsRNA) and two other commercial enzymes used for producing siRNAs (from major suppliers) were cleaved using the PowerCut Dicer. Reactions were performed according to the manufacturers’ instructions. PowerCut Dicer cleaved all the three substrates with 100% efficiency. The conventional dicer gave only partial digestion, whereas RNase III also digested part of the produced siRNAs resulting in lower yield. M denotes a 27 bp RNA marker.
 

Efficient silencing in HeLa cells

The HeLa-eGFP cell line containing constitutively expressed pEGFP-C1 plasmid was transfected with non-specific control siRNAs (Phi6 S) and specific eGFP siRNAs produced with the PowerCut Dicer. The results show that specific siRNAs efficiently silenced the expression of eGFP, whereas the non-specific control siRNA has no effect. The eGFP siRNAs were cleaved from a dsRNA substrate produced with the Replicator RNAi Kit.

原厂资料：