Thermo Scientific Direct PCR enables PCR directly from source materials without prior DNA purification. The best results are obtained by using a very small amount of sample as starting material. The Harris Uni-Core™ is a convenient tool for cutting very small punch discs from materials such as animal tissue, plant leaves or Whatman FTA or Whatman 903 cards for Direct PCR. A compatible Harris Cutting Mat™ is used to provide an even cutting surface for the Harris Uni-Core™.
Steps for taking a sample with the Harris Uni-Core™
•Step 1 – While holding the puncher firmly, press the cutting edge downward into the tissue and rotate the puncher between your fingers in opposite directions.
•Step 2 – Press the plunger to eject the punch disc into a PCR reaction. Make sure that the sample drops into the PCR solution and does not stick into the tube walls.
•Step 3 – To prevent cross-contamination, it’s important to clean the cutting edge of the puncher between each sample by dipping it into 2% NaClO solution. Press the plunger up and down a few times.
•Step 4 – Wipe the cutting edge with a clean paper towel. Remember to rinse the cutting mat as well.
The steps can be repeated for additional samples.
Direct PCR protocols using the Harris Uni-Core™
•Technical Manual for Phire Animal Tissue Direct PCR Kit
•Technical Manual for Phire Plant Direct PCR Kit
•Application Note: Avoiding cross-contamination when using the Harris Uni-Core™
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注意事项：
Harris Uni-Core™ and Harris Cutting Mat™ are trademarks of Shunderson Communications Inc.
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