Thermo Scientific ABsolute qPCR Mixes are optimized for use with hybridization probes and contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X master mix contains a proprietary reaction buffer that provides high sensitivity, specificity, and reproducibility. ABsolute qPCR Mixes are available with the option of included high ROX reference dye concentration, Low ROX concentration, and with no ROX reference dye. There is also a master mix version optimized for capillary instruments.
Highlights
High sensitivity & specificity - The master mixes contain our patented hot start enzyme Thermo-Start DNA Polymerase
Reproducibility - All mixes undergo extensive QC to ensure batch to batch consistency
Optimised buffer - For maximal performance in multiplex reactions
Applications
Gene expression analysis
siRNA validation
Genotyping
Absolute qPCR Mix (no ROX) contains 2X Master Mix and no passive reference dye.